Two genes were then expressed in Escherichia coli, and the recombinant proteins showed strong binding to female pheromone components in fluorescence-binding assays. In an analysis of the differentially expressed genes in the antennae of males under red light and dark conditions, the expression levels of two odorant-binding protein (OBP) genes, CpunOBP2 and CpunPBP5, were up-regulated. In addition, the electroantennografic response of males against the female sex pheromone increased with red light treatment during scotophase. Under the dim red light, the enhancement of mating in C. To test the hypothesis, the mating of moths under dim red, blue, and white lights during scotophase was observed. In this study, on the basis of long-term insects rearing experience, dim red light (spectrum range: 610–710nm, with a peak at 660nm 2.0 Lux) during scotophase was hypothesized to enhance mating in the yellow peach moth, Conogethes punctiferalis. Although a few studies examine changes in insect mating under artificial light at night, detailed influences of light, such as that of monochromatic light, on moth mating remain largely unknown. Moth copulation and mating usually occur at night. Light at night has important influences on nocturnal insects, including most moth species. Insects are behaviorally and physiologically affected by different light conditions, including photoperiod, light intensity, and spectrum. 3State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Southwest University, Chongqing, China.2Academy of Agricultural Sciences, Southwest University, Chongqing, China.1Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
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